The Combined Effect of Berberine and Quercetin against Non-Alcoholic Fatty Liver Disease

Abstract

The aim of the present study was to explore the protective effect of Berberine and Quercetin against non-alcoholic fatty liver disease. The phrase "non-alcoholic fatty liver disease" refers to a number of liver disorders that can afflict persons who use little to no alcohol. There is currently no effective treatment for NAFLD, and the underlying molecular mechanism that causes this transformation is unclear. Growing interest has been shown in developing natural compound-based therapeutics for metabolic illnesses. Natural supplement berberine is well known for its effective Antibacterial, Antiprotozoal, and Antitrachoma properties in addition it has Hepatoprotective. An essential natural Flavonoid called quercetin has attracted medical interest because of its possible positive effects on human health. It can reduce blood cholesterol levels, inflammation, reduce hepatic fat build up, and regulate abnormalities of the gut microbiota. The MTT test was used to determine if the combination of the substances was more effective against NAFLD than either berberine or quercetin taken separately.HepG2 cells were exposed to a combination of BBR and quercetin at various doses in five different concentration .This study's findings comes to the conclusion that quercetin and berberine together had demonstrated efficacy against cell lines. The combination, individual chemicals, and Rf value of berberine and quercetin all shown to be genuine. The FTIR measurement ultimately demonstrated that there is no herbal- herbal interaction. Therefore, further research is required before moving forward with in vivo investigations using animal models and developing the medicine for clinical assessment before marketing the herbal product for NAFLD.